This items is made to order and is not returnable and order cannot be canceled.

CUSTOM ITEM: Guard Column cartridge, Bidentate C8, replacement, 4.0 mm ID x 10 mm long cartridge packed with 5 um, 300 A phase. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 40008‑PG6‑3 Cogent Bidentate C8 Guard Column Cartridge is a single replacement cartridge designed to protect standard‑flow analytical HPLC columns during wide‑pore (300 Å) separations, especially for larger molecules and complex sample matrices.

Each cartridge is:

  • 4.0 mm ID × 10 mm length

  • Packed with 5 µm, 300 Å TYPE‑C™ silica hydride Bidentate C8 phase

  • Constructed in stainless steel housing with SS frits

The cartridge is supplied in an individual protective Delrin™ case, preventing contamination, drying, and physical damage prior to use.

⚠️ Requires guard column holder (sold separately)

Designed for Standard Analytical Column IDs

This cartridge is optimized for:

  • 3.0 mm ID solvent‑saving HPLC columns

  • 4.0 mm ID analytical columns

  • 4.6 mm ID standard analytical HPLC columns

Ideal for traditional HPLC systems, it ensures:

  • Minimal band broadening

  • Maintained chromatographic efficiency

  • Reliable and reproducible performance

Why Use a Single Guard Cartridge

A single‑cartridge format is ideal when:

  • Evaluating different guard phases during method development

  • Pairing multiple stationary phases with different analytical columns

  • Replacing cartridges individually as needed

  • Maintaining flexibility without purchasing multi‑packs

This format supports custom method optimization and workflow flexibility.

Why Use 300 Å Guard Cartridges

The 300 Å pore size is specifically designed for:

  • Peptides and proteins

  • Large molecular weight analytes

  • Complex or biological sample matrices

Compared to 100 Å phases:

  • Improves accessibility for large molecules

  • Reduces steric exclusion effects

  • Enhances recovery and reproducibility for biomolecule separations

Why Use Guard Column Protection

Analytical columns are routinely exposed to:

  • Particulates and insoluble debris

  • Strongly retained compounds

  • Sample matrix buildup

  • Irreversible adsorption

The 40008‑PG6‑3 cartridge acts as a sacrificial protection layer, helping to:

  • Extend analytical column lifetime

  • Maintain consistent chromatographic performance

  • Reduce downtime and replacement costs

  • Improve reproducibility across analyses

Cartridges can be replaced quickly without disturbing the analytical column.

Bidentate C8 Guard Phase Advantages

Matching the guard phase to your analytical column ensures:

  • Consistent selectivity and retention behavior

  • No unexpected method changes

  • Seamless analyte transfer

Compared to C18 phases, Bidentate C8:

  • Reduces excessive hydrophobic retention

  • Improves recovery of amine‑containing analytes

  • Provides alternative selectivity for complex samples

  • Supports faster equilibration

Cogent TYPE‑C™ Technology Benefits

Silica Hydride Surface

  • Reduced silanol interactions

  • Improved peak shape for basic and polar analytes

  • Lower secondary interactions

Faster Equilibration

  • Reduced run‑to‑run cycle times

  • Increased laboratory throughput

Durability

  • Resistant to hydrolysis

  • Long cartridge lifetime

  • Stable under demanding analytical conditions

Typical Applications

  • LC‑MS and HPLC biomolecule analysis

  • Peptide and protein separations

  • Pharmaceutical and bioanalytical workflows

  • Complex biological matrices

  • Wide‑pore chromatography methods

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

  • Orders cannot be canceled once placed

  • Custom items are non‑returnable

Custom Order Item. Inquire for more information.

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